The National League for Democracy "expressed concern" that Suu Kyi has not accepted food delivered to her home for almost three weeks, the party said in a statement.
It did not say whether she was on a hunger strike, a question that has remained unanswered since the first mention of her refusal to accept food over a week ago.
The 63-year-old Nobel Peace Prize laureate has been held in detention by the ruling military junta for 13 of the past 19 years, mostly under house arrest, and relies on food delivered by her party for sustenance.
Friday's statement called Suu Kyi's action a protest, which had only been alluded to until now.
"She is refusing food supplies in protest against ... her unlawful detention under the security law," the party said.
Suu Kyi also wants greater freedom of movement for two female companions who live with her and help take care of the house, it said. They are currently not allowed to leave the compound.
She is also protesting that authorities have not allowed her to receive a monthly medical checkup by her physician as they earlier promised, it said. A doctor visited Suu Kyi on Aug. 17, but her previous checkup was in January, the party said.
"Her safety and well-being are the soul responsibility of the authorities who have unlawfully detained her," it said.
Suu Kyi's lawyer, Kyi Win, was allowed to meet with her for 30 minutes on Monday, and said she told him that "I am well but I have lost some weight."
Rumors of a possible hunger strike have circulated widely in Yangon, where Suu Kyi's isolation has only increased the mystique that surrounds her.
Similar hunger strike rumors spread in 2003 and in 1989, but proved untrue.
Supporters have speculated that Suu Kyi is frustrated over the United Nations' failure to bring about democratic reform in the country, which has been ruled by the military since 1962.
Suu Kyi canceled meetings with U.N. special envoy Ibrahim Gambari during his six-day visit to Myanmar last month, and he left without seeing her.
U.N. envoys and other senior officials have visited the country nearly 40 times since 1990, and the U.N. General Assembly has passed numerous resolutions calling for change.
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